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KMID : 0604520210470010057
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Journal of the Society of Cosmetic Scientists of Korea
2021 Volume.47 No. 1 p.57 ~ p.63
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Analytical Method Development of Isoscoparin in Silene seoulensis Extract Using HPLC
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Kwon Jin-Gwan
Seo Chan-Gon
Jung Yeon-Woo
Choi Yong-Mun
Shin Hyun-Tak
Jung Su-Young
Choi Jeong-June
Kim Jin-Kyu
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Abstract
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In this study, isoscoparin was selected as an indicator component to develop Silene seoulensis extract as a functional cosmetic material, and we developed an analysis method using high performance liquid chromatography (HPLC) for quality control. HPLC was performed on a Unison US-C18 with a gradient elution of 0.05% (v/v) trifluoroacetic acid (TFA) and methanol at a flow rate of 1.0 mL/min at 35 ℃, and the detection wavelength was 330 nm. The HPLC method was performed in accordance with the international conference on harmonization (ICH) guideline (version 4, 2005) of analytical procedures with respect to specificity, precision, accuracy, and linearity. The limits of detection and quantitation were 0.02 and 0.07 mg/mL respectively. Calibration curves showed good linearity (R2 > 0.99988), and the precision of analysis was satisfied (less than 0.46%). In addition, the recovery rate was in the range of 99.10 to 101.61%, it was shown to be accurate. This result indicated that the established HPLC method is very useful for the determination of marker compounds in Silene seoulensis extracts.
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KEYWORD
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silene seoulensis, isoscoparin, functional cosmetic, HPLC, validation
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